The Effect of Cryopreservation on Cytochrome Oxidase1 (CO1) Gene and the Relationship with Spermatozoa Motility of Albino Pangasius catfish (Pangasionodon hypophthalmus)

Cryopreservation is a technique for storing cells and tissues at very low temperatures the possible usage of stored throughout year. Sperm cell cryopreservation in some species causes decrease sperm quality DNA damage. Inappropriate protocols can cause changes physiology. Mitochondria are organelles that play role producing energy motility. have molecules with small sizes compared to structures from nuclear DNA. This study analyzed effect on motility Cytochrome Oxidase1 (COI) gene. The CO1 gene mitochondrial plays production spermatozoa was performed using skim milk 10% methanol cryoprotectant, temperature equilibration process 4-5°C 10 minutes. took place 14 days freezer -80°C. In addition, thawing 1-2 minutes 40°C. found number lesions per kb post-equilibration (9.24±3.74), post-thawing (was 10.26±7.54). Spermatozoa obtained fresh spermatozoa, i.e., 87±1.5%, 79±4.5%, 30±3.2%. concluded cryopreserved there spermatozoa.